《植物生理学报》 2015, 51(9): 1513-1523

高粱SbHKTs基因的克隆及其在拟南芥中的功能验证

冯雪^1,2,*, 王甜甜^2,3,*, 郝怀庆², 邹春静^1,**, 景海春^2,**

¹华东师范大学生命科学学院, 上海200062; ²中国科学院植物研究所北方资源植物重点实验室, 北京100093; ³东营市园林局, 山东东营257091

通信作者：冯；E-mail: cjzou@bio.ecnu.edu.cn, hcjing@ibcas.ac.cn；Tel: 021-6223359, 010-62836576

摘　要：

本试验克隆了高粱HKT (high affinity potassium transporter)基因家族3个成员SbHKT1;4, SbHKT1;5和SbHKT2;1, 并分析比较了这些基因在拟南芥中转运钠、钾离子的特性以及在抗旱方面的作用。结果表明, 外界高浓度钠胁迫对各转基因植株的生长均有一定的影响, 但这种抑制作用可在外源添加足量钾离子的情况下得到不同程度的缓解。此外，干旱胁迫下，转基因植株表现出优于突变体的抗旱性。这些结果表明，高粱HKT基因不仅在作物耐盐方面具有重要作用, 同时在植物抗旱中也具有一定的功能。

关键词：高粱HKT基因; 拟南芥; 耐盐性; 抗旱性

收稿：2015-04-17   修定：2015-08-24

资助：国家科技支撑计划(2013BAD22B01和2015BAD15B03)和青年科学基金项目(31301326)。 * 共同第一作者。

Cloning of Sorghum HKT Genes and Their Function Validation in Arabidopsis

FENG Xue^1,2,*, WANG Tian-Tian^2,3,*, HAO Huai-Qing², ZOU Chun-Jing^1,**, JING Hai-Chun^2,**

¹School of Life Sciences, East China Normal University, Shanghai 200062, China; ²Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; ³Dongying Landscape and Greening Department, Dongying, Shandong 257091, China

Corresponding author: FENG Xue; E-mail: cjzou@bio.ecnu.edu.cn, hcjing@ibcas.ac.cn; Tel: 021-6223359, 010-62836576

Abstract:

Three sorghum high affinity potassium transporter genes, SbHKT1;4, SbHKT1;5 and SbHKT2;1, were cloned and constitutively expressed in Arabidopsis hkt1-1 mutant, respectively, and the tolerance to salt and drought of the transgenic lines analyzed. Upon high Na⁺ stress, the growth of all transgenic lines was inhibited, and this phenotype could be obviously alleviated when adequate K⁺ was added. After water stress, we found that transgenic lines were more tolerant to drought than athkt1-1 mutant line. These results indicated that SbHKTs not only played a role in tolerance to salt stress, but also exerted significant function in the presence of drought-stress.

Key words: sorghum HKT; Arabidopsis; salt resistance; drought tolerance